This invention relates to methods of generating single stranded DNA libraries for use in amplification and sequencing reactions. In various aspects, the disclosed methods include: fragmenting DNA; polishing the fragments' ends; lighting the fragments to universal adapters; performing strand displacement and extension of the nicked fragments; purifying the double-stranded libation products; capturing the double-stranded libation products onto a solid support; and isolating single stranded DNA library fragments, and binding these fragments to another solid support.
申请公布号
DE602004026033(D1)
申请公布日期
2010.04.29
申请号
DE20046026033T
申请日期
2004.01.28
申请人
454 CORP.
发明人
CHEN, YI-JU;LEAMON, JOHN H.;LOHMAN, KENTON;RONAN, MICHAEL T.;SRINIVASAN, MAITHREYAN;ROTHBERG, JONATHAN;WEINER, MICHAEL