PROCESS FOR PRODUCING L- AND D-ALIPHATIC AMINO ACID HYDROXIDES

摘要

Disclosed are: an aliphatic amino acid hydroxylase having low substrate specificity; and a process for producing a hydroxide of an aliphatic amino acid by using the enzyme, which can produce a hydroxide of any one of various aliphatic amino acids by changing a substrate molecule for the enzyme. Specifically disclosed are: an L- or D-aliphatic amino acid dioxygenase which can catalyze the hydroxylation reaction of an L- or D-form of an aliphatic amino acid in the presence of 2-oxoglutaric acid and a bivalent iron ion; and a process for producing a hydroxide of an L- or D-aliphatic amino acid.  The L- or D-aliphatic amino acid dioxygenase may be a protein comprising an amino acid sequence depicted in SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6 or SEQ ID NO:8.  The process for producing a hydroxide of an L- or D-aliphatic amino acid comprises a step of allowing the L- or D-aliphatic amino acid dioxygenase to act on an aliphatic amino acid to produce a hydroxide of the aliphatic amino acid.