发明名称 |
ASSAY WITH REDUCED BACKGROUND |
摘要 |
In an assay, an analyte is specifically associated with a reporter adenylate kinase, ADP is added and then formation of ATP is mo nitored. Prior to addition of ADP, adenylate kinase other than reporter adenylate kinase is removed. Assay apparatus comprises a solid phase on which is immobilised the analyte or an antibody specific for the analyte, a reporter composition comprising a thermostable adenylate kinase coupled to an antibody specific for the analyte, and ADP plus associated reagents for conversion of ADP into ATP by thermostable adenylate kinase. |
申请公布号 |
CA2360779(C) |
申请公布日期 |
2010.04.27 |
申请号 |
CA20002360779 |
申请日期 |
2000.02.03 |
申请人 |
MICROBIOLOGICAL RESEARCH AUTHORITY |
发明人 |
RAVEN, NEIL DAVID HAMMOND;WICTOME, MATTHEW PATRICK |
分类号 |
C12N9/12;G01N33/543;C12Q1/34;C12Q1/48;C12Q1/66;G01N33/535;G01N33/58 |
主分类号 |
C12N9/12 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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