发明名称 |
METHOD FOR GENERATING STABLE CELL LINES EXPRESSING HIGH LEVELS OF A PROTEIN OF INTEREST. |
摘要 |
<p>This invention relates to industrial production of proteins. More specifically, the invention relates to a method for obtaining cells that stably express a protein of interest, even when cultivated in the absence of selective pressure. DHFR is used as a surrogate marker. The transfected cells are not selected based on resistance to a toxic compound, but based on fluorescence as measured by FACS using fluorescent MTX.</p> |
申请公布号 |
MX2010000614(A) |
申请公布日期 |
2010.04.22 |
申请号 |
MX20100000614 |
申请日期 |
2008.07.16 |
申请人 |
MERCK SERONO SA |
发明人 |
CRAWFORD MC FADD, TARA KRISTEN;YANG, MEIJIA |
分类号 |
C12N15/65;C12N15/67;C12N15/79 |
主分类号 |
C12N15/65 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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