发明名称 |
Allele-Specific Amplification |
摘要 |
The present invention includes a method of allele-specific amplification, utilizing an allele-specific oligonucleotide, at least partially complementary to more than one variant of the target sequence, but having a 3′-terminal nucleotide complementary to only one variant of the target sequence and having at least one nucleotide with a base covalently modified at the exocyclic amino group, wherein the allele-specific oligonucleotide is extended by a nucleotide-incorporating biocatalyst predominantly when hybridized to the variant of the target sequence for which it has said complementary 3′-terminal nucleotide.
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申请公布号 |
US2010099110(A1) |
申请公布日期 |
2010.04.22 |
申请号 |
US20090582068 |
申请日期 |
2009.10.20 |
申请人 |
ROCHE MOLECULAR SYSTEMS, INC. |
发明人 |
WILL STEPHEN G.;TSAN ALISON;NEWTON NICOLAS |
分类号 |
C12Q1/68;C07H21/00;C12P19/34 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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