| 摘要 |
<p>A method and apparatus for analyzing compounds with amino group including various amino acids, derivatives thereof, and amino acid analogues contained in biological organisms far more rapidly and easily with higher sensitivity than conventional methods. A compound with amino group is reacted with a reagent for derivatization to generate a derivative of the compound with amino group represented by the following general formula (I), and then the derivative of the compound with amino group is eluted by liquid chromatography using a stepwise elution means on a concentration gradient. Subsequently, the derivative of the compound with amino group eluted from the liquid chromatography is detected by mass spectrometry.
In the formula, Ar 1 denotes an optionally substituted hydrocarbon, or a substituent comprising a carbocyclic or heterocyclic ring having an aromaticity, R 1 denotes a hydrogen atom, an optionally substituted alkyl group, or a ring forming carbon atom, and R 2 denotes an optionally substituted alkyl group, where Ar 1 and R 1 , or R 1 and R 2 may form a ring with each other.</p> |
