摘要 |
The invention relates to a regulation system for inducible expression of genes, comprising a lambdoid promoter, a gene coding for a repressor for the lambdoid promotor and a gene coding for an antirepressor of the repressor, which antirepressor is under the influence of an inducible promotor. The invention further relates to a regulatory replicon, comprising said gene coding for an antirepressor, an expression system, comprising said regulatory replicon, and an expression vector based on a lambdoid promotor, and also to a method for producing a gene product in a heterologous host, by providing a culture of a host comprising a heterologous sequence which codes for the gene product, wherein the expression of the heterologous sequence is under the control of a regulation system, a gene coding for a repressor for the lambdoid promotor and a gene coding for an antirepressor, and by inducing the promotor of the antirepressor gene.
|