发明名称 NEUTRAL PROTEASE AND PRODUCT FOR TISSUE DISSOCIATION USING NEUTRAL PROTEASE AND METHOD OF PRODUCTION THEREOF
摘要 <p><P>PROBLEM TO BE SOLVED: To provide products for tissue dissociation, obtaining isolation results improved in yield, viability and integrity of the cells, to provide a method of production thereof, and to provide use thereof. <P>SOLUTION: The use of a neutral protease (NP) together with a collagenase consists in that a neutral protease, which is not contained in a collagenase enzyme preparation and which is not produced by a recombinant production, is mixed, before the beginning of a tissue dissociation, with a collagenase or a collagenase enzyme preparation with an individual dosage of the quantitative proportions of neutral protease and collagenase, for improving the isolation results with respect to yield, viability and integrity of the cells. <P>COPYRIGHT: (C)2010,JPO&INPIT</p>
申请公布号 JP2010081941(A) 申请公布日期 2010.04.15
申请号 JP20100005801 申请日期 2010.01.14
申请人 NORDMARK ARZNEIMITTEL GMBH & CO KG 发明人 KURFUERST MANFRED;RAEMSCH CHRISTIAN;RAEMSCH-GUENTER NICOLE;FRIEDRICH OLAF;HUETTLER SILKE;BRANDHORST DANIEL;BERNEY THIERRY;BUCHER PASCAL
分类号 C12N9/50;C12N15/09;C12N5/071;C12N9/52;C12N9/64 主分类号 C12N9/50
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