LINKING PROTEIN AGGREGATION AND YEAST SURVIVAL

摘要

The invention relates to methods for the identification of compounds capable of preventing the aggregation of aggregation-prone polypeptides based on the use of fusion proteins which comprise an aggregation-prone polypeptide and an enzyme which is able to metabolize a compound showing toxicity of the cell whereby if the compound prevents aggregation of the aggregation-prone polypeptide, the enzyme will remain soluble and in active form thus preventing the toxic effect of the compound on the cell and increasing cell viability. The invention also provides polypeptides comprising an aggregation-prone polypeptide and an enzyme and uses thereof.