摘要 |
Apparatus for electrophoretic transfer of nucleic acids, peptides, proteins and/or other biologically active molecules into eukaryotic cells comprises two capacitors, each connected to a high voltage source. - Apparatus for electrophoretic transfer of nucleic acids, peptides, proteins and/or other biologically active molecules into eukaryotic cells comprises two capacitors (7, 8), each connected to a high voltage source (5, 6). Each capacitor discharges via a power transistor (9, 10) into a cuvette holding the cell sample. The first capacitor is charged with a preset voltage U1 and the second with a voltage U2 given by the equation: - U2 = R x I2 x K2, - R1 = the resistance of the cuvette and suspension in it - I2 = the desired current - K2 = a correction factor based on the properties of thee cuvette - ACTIVITY - None given. - MECHANISM OF ACTION - Gene therapy. - No supporting data provided. |