HOLOGRAPHIC SENSOR AND ITS USE IN THE DETECTION OF ENZYME INHIBITION

摘要

A sensor for an analyte which affects an enzyme-catalysed reaction is described, in which the sensor comprises a first layer of a hydrogel containing the enzyme and a second layer comprising a polymer matrix in which a hologram is written and is sensitive to a product of the reaction. Such a sensor can be used, for example, in a method for determining activity of the enzyme, by contacting a sample containing a substrate of the enzyme with the sensor, and observing any change in the hologram.