MULTIPLEX AMPLIFICATION OF SHORT TANDEM REPEAT LOCI

摘要

PROBLEM TO BE SOLVED: To provide methods simultaneously amplifying at least thirteen loci of genomic DNA in a single multiplex reaction.

SOLUTION: The method comprises: a step of selecting at least one DNA sample to be analyzed comprising at least thirteen short tandem repeat loci which can be co-amplified; a step of co-amplifying the loci in the set in a multiplex amplification reaction, wherein the product of the reaction is a mixture of amplified alleles from each of the co-amplified loci in the set; and a step of evaluating the amplified alleles in the mixture to determine the alleles present at each of the loci analyzed in the set within the DNA sample. The invention includes materials used in the method.

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