GENOME-SCALE BUTANOL-PRODUCING MICROORGANISM METABOLIC NETWORK MODEL AND METHODS USING THE SAME FOR ANALYSIS OF METABOLIC CHARACTERISTICS OF BUTANOL-PRODUCING MICROORGANISMS AND FOR SCREENING DELETION TARGETS

摘要

The present invention relates to a metabolic network model for analysis of metabolic characteristics of butanol-producing microorganisms and methods using the same for analysis of metabolic characteristics of butanol-producing microorganisms and for screening deletion targets; and, more particularly, to establishing a metabolic network model of butanol-producing microorganisms using gene-protein-biochemical reaction associations and methods for analysis of metabolic characteristics such as a metabolic flow, etc., using the established model and for screening deletion targets with a simulation based on the metabolic flow analysis to improve the productivity of a specific metabolic product during the deletion.  A metabolic network model according to the invention is useful for analysis of metabolic characteristics such as a metabolic flow, etc. of butanol-producing microorganisms and for screening a deletion target enzyme or a gene therefor to increase the production of a specific metabolic product.  Moreover, unlike conventional methods, a deletion target screening method according to the invention makes it possible to predict deletion targets in an efficient manner, to save time and costs and to provide mutant microorganisms capable of producing a specific metabolic product at a high efficiency.