Microbiological production of 3-hydroxypropionic acid

摘要

Genetically modified cells, exhibiting an increased activity of an enzyme E 1a that catalyzes the conversion of pyruvate to oxaloacetate, or of an enzyme E 1b that catalyzes the conversion of phosphoenol pyruvate to oxaloacetate; an increased activity of an enzyme E 2 that catalyzes the conversion of aspartate to beta alanine; an ability to lock-out beta alanine from the cell and/or an ability to convert beta alanine to 3-hydroxypropionic acid, with respect to its wild-type, are new. Independent claims are included for: (1) production of genetically modified cells; (2) production of 3-hydroxy propionic acid comprising contacting the genetically modified cells that exhibits an ability to lock-out beta alanine from the cell and/or an ability to convert beta alanine to 3-hydroxypropionic acid, with a growth medium containing carbohydrates or glycerin under the conditions that form beta alanine from carbohydrates or glycerin, where the produced beta alanine reaches the growth medium to form a growth medium containing beta alanine; contacting the growth medium containing beta alanine with a further cell that is capable to take up and convert the beta alanine to 3-hydroxy propionic acid; (3) production of acrylic acid, comprising producing 3-hydroxy propionic acid by the above method; and dehydrating the 3-hydroxy propionic acid; (4) a method for the production of polyacrylates comprising producing 3-hydroxy propionic acid by the above method, dehydrating the 3-hydroxy propionic acid under formation of acrylic acid and radically polymerizing the acrylic acid; and (5) production of acrylic acid esters comprising producing 3-hydroxy propionic acid by the above method, dehydrating the 3-hydroxy propionic acid under formation of acrylic acid and esterifying the acrylic acid.