发明名称 |
ACETYL-COA PRODUCING ENZYMES IN YEAST |
摘要 |
The present invention relates to a method of identifying a heterologous polypeptide having enzymatic activity for converting pyruvate, acetaldehyde or acetate into acetyl-CoA in (the cytosol of) a yeast cell comprising: a) providing a mutated yeast cell comprising a deletion of at least one gene of the (PDH) by-pass, selected from the genes encoding the enzymes pyruvate decarboxylase (PDC), acetaldehyde dehydrogenase (ALD), and acetyl-CoA synthetase (ACS); b) transforming said mutated yeast cell with an expression vector comprising a heterologous nucleotide sequence encoding a candidate polypeptide having potential enzymatic activity for converting pyruvate, acetaldehyde or acetate into acetyl-CoA; c) testing said recombinant mutated yeast cell for its ability to grow on minimal medium containing glucose as sole carbon source, and d) identifying said candidate polypeptide as a heterologous polypeptide having enzymatic activity for converting pyruvate, acetaldehyde or acetate into acetyl-CoA in (the cytosol of) said yeast cell when growth of said cell is observed. The invention further relates to a method of producing a fermentation production such as butanol. |
申请公布号 |
EP2173881(A2) |
申请公布日期 |
2010.04.14 |
申请号 |
EP20080775033 |
申请日期 |
2008.07.11 |
申请人 |
DSM IP ASSETS B.V. |
发明人 |
MUELLER, ULRIKE, MARIA;WU, LIANG;RAAMSDONK, LOURINA, MADELEINE;WINKLER, AARON ADRIAAN |
分类号 |
C12P7/16;C07K14/33;C12N9/02;C12Q1/32 |
主分类号 |
C12P7/16 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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