发明名称 |
FLUORESCENCE LIFETIME AND FLUORESCENCE ASSAYS |
摘要 |
The invention provides a method for determining a degree of phosphorylation of a substrate, for example a peptide substrate, using a fluorescence probe that acts alone or with another material and has a lifetime that varies when in proximity to a phosphate, the method comprising: causing the fluorescence probe to fluoresce; measuring a time response of the fluorescence, and analysing the fluorescence time response to identify a fluorescence component having a lifetime associated with phosphorylated substrate and a fluorescence component having a lifetime associated with un-phosphorylated substrate. |
申请公布号 |
EP2174138(A2) |
申请公布日期 |
2010.04.14 |
申请号 |
EP20080762446 |
申请日期 |
2008.06.20 |
申请人 |
EDINBURGH INSTRUMENTS LIMITED;THE UNIVERSITY COURT OF THE UNIVERSITY OF DUNDEE |
发明人 |
GRAY, ALEXANDER;PATERSON, MICHAEL, JOHN;GAKAMSKY, DMITRY |
分类号 |
G01N33/58;C12Q1/42;C12Q1/48 |
主分类号 |
G01N33/58 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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