| 摘要 |
FIELD: agriculture. ^ SUBSTANCE: set of oligonucleotides-primers is proposed for identification of virus of raspberry bushy dwarf comprising 2 pairs of desoxyoligonucleotides having activity of direct and reverse primers in reaction of reverse transcription and polymerase chain reaction. Primers have the following structure: 5'ttcttttgtcgggttcagtgag3' - RBDV-CP+2; 5'aactattgtggaggatttgc3' - RBDV-CP-2, 5'gacatgtatatgtctgctaagg3' - RBDV-CP+3; 5'tgtcgtcgacggcaccgccc3' - RBDV-CP-3. According control oligonucleotides are also available for set of primers. Usage of primers set makes it possible to detect VKKM virus in the territory of Russia. ^ EFFECT: invention may be used in biotechnology of plants, phytopathology, selection, berries and plants growing for detection of genetic material of raspberry bushy dwarf virus in samples of test-tube and field plants. ^ 3 dwg, 1 tbl, 2 ex |
