STRAIN OF HYBRID CULTIVATED MURINE CELLS Mus Musculus-6F3 PRODUCING MONOCLONAL ANTIBODY NONTOLERANT TO HEMAGGLUTININ PROTEIN OF HIGH-PATHOGEN STRAIN virus A/duck/Novosibirsk/56/05 H5N1

摘要

FIELD: medicine. ^ SUBSTANCE: invention aims at preparation of new strain of hybrid cells Mus. Musculus 6F3 - a producer of monoclonal antibody (MCA) to hemagglutinin protein of high-pathogen avian influenza virus A/duck/Novosibirsk/56/05. Strain 6F3 is prepared by fusing murine myeloma cells Sp2/0 with murine spleen cells BALB/c, immunised with a purified and inactivated preparation of avian influenza virus A/H5N1 (strain A/duck/Novosibirsk/56/05). Hybridoma produced MCA belong to IgA class. Strain 6F3 is deposited in the Collection of cell culture of Ivanovsky State Research Institution of Virology of the Russian Academy of Medical Sciences, No. 8/2/3. Using hybridoma allows producing specific monoclonal antibodies to hemagglutinin protein of avian influenza virus A/H5N1. ^ EFFECT: possibility to use antibodies to studying the antigenic structure of hemagglutinin for differential diagnostics of avian influenza virus A/H5 serotype. ^ 1 dwg, 6 ex