| 摘要 |
PURPOSE: A kit for identifying or detecting synovial membrane cells with reduced proliferation or chondroplasia ability is provided to easily and accurately identify chodrocytes and treat injury of joint cartilage. CONSTITUTION: A kit for identifying or detecting synovial membrane cells with reduced proliferation or chondroplasia ability contains a primer or probe which specifically binds to cyclin-dependent kinase inhibitor 2A gene, v-Ki-ras 2 gene, thrombospondin 1 gene, mitogen-activated protein kinase 9 gene, ecto-nucleotide pyrophosphatase/phosphodiesterase 1 gene, tumor necrosis factor receptor superfamily member 6 gene, tumor necrosis factor receptor superfamily member 10d gene, tumor necrosis receptor superfamily member 10b gene, tumor necrosis factor receptor superfamily 11b gene, TNF receptor-related factor 4 gene or PRKC apoptosis WT1 regulator(PAWR) gene sequence. A method for identifying or detecting synovial membrane cells with reduced proliferation or chondroplasia ability comprises: a step of collecting total RNA from synovial membrane cells; a step of constructing cDNA from the total RNA; a step of hybridizing the cDNA with specific probe; or a step of detecting hybridization, a step of amplifying cDNA using specific primer; and a step of confirming amplified product.
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