BALANCED-QUADRATURE INTERFEROMETRIC PROTEIN MICROARRAY

摘要

A land contrast platform for interferometrically detecting the presence of a target analyte in a sample is disclosed. The land contrast platform includes a spatially patterned substrate with a layer of recognition material immobilized thereon. The spatially patterned substrate includes a plurality of high regions and low regions; the high regions being at a substantially uniform first height and the low regions being at a substantially uniform second height, the first and second heights being different. The layer of recognition material is designed to bind with the target analyte. The high and low regions have approximately the same reflectance but complementary quadrature conditions such that equal target analyte binding on the high and low regions produces opposite responses; target binding on the high regions producing a positive or negative reflectance difference and target binding on the low regions producing the other of the positive or negative reflectance difference.