| 摘要 |
A homogenous bioassay including i) a first group containing a short lifetime fluorescent acceptor capable of energy transfer, and ii) a second group containing a quencher capable of energy transfer from an acceptor, with the first and second groups linked by at least a first linkage. The bioassay measures the acceptor's fluorescence increase resulting from cleavage of the first linkage. The bioassay also includes iii) a third group containing a donor for energy transfer to the acceptor, where the donor is an up-conversion fluorescent compound, a long-lifetime fluorescent compound or an electrogenerated luminescent compound. A conformational or terminal epitope is created on the first group through cleavage of the linkage, and the third group includes a binder with affinity for this conformational or terminal epitope. The acceptor's fluorescence is brought about by exciting the donor. Also disclosed are kits for homogenous bioassays according to the method of the invention.
|
