摘要 |
<p>A probe array device, method, and system for detecting intergenic fusions between a selected pair of genes in the same or different chromosomes are disclosed. The array device provides a plurality of exon-exon boundary spanning probes whose sequences span each of the possible combinations of illegitimate exon-exon boundaries, in both gene fusion transcript directions, for a selected gene pair. Exon-exon boundary spanning probes having elevated test/mean test and/or test/reference/mean test/reference values are identified as candidate exon-exon boundaries for a gene fusion, and these events are confirmed against exonic and intronic probes in the device that provide positive and negative controls, respectively.</p> |