| 摘要 |
A liquid chromatograph includes a mobile phase supplying apparatus including a plurality of liquid-feeding pumps to feed mobile phases, a plurality of liquid-feeding flow paths, a mixer to mix the mobile phases by merging the liquid-feeding flow paths, at least one control device to control driving of each of the liquid-feeding pumps based on flow rates for the respective liquid-feeding flow paths, a plurality of flow rate measuring sections provided downstream of the liquid feeding pump in each liquid-feeding flow path, the plurality of actual flow rate measuring sections configured to measure an actual flow rate and to detect a back-flow in the corresponding liquid-feeding flow path, and a plurality of actual flow rate computing sections configured to compute an actual flow rate in the corresponding liquid feeding flow path. The liquid chromatograph also includes a sample injecting section downstream of the mobile phase supplying apparatus where the mobile phases are supplied from the mobile phase supplying apparatus, a separating column for provided downstream of the sample injecting section for separating an injected sample into its constituents, and a detector for detecting each of the separated constituents. When one of the plurality of actual flow rate measuring sections detects a back-flow in the corresponding liquid-feeding flow path, the corresponding actual flow-rate computing section computes an actual back-flow rate and outputs a signal to the corresponding control device which controls the corresponding liquid-feeding pump so as to cancel the back-flow based on the computed actual flow rate.
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