FLUORESCENCE BASED DETECTION METHODS AND APPARATUS

摘要

Apparatus for detecting fluorescence emitted from samples in multiple reaction vessels in response to excitation by a radiation source. Two or more radiation sources irradiate the samples within the multiple reaction vessels with excitation radiation over the same time period. A modulation arrangement modulates the excitation radiation of each of the sources according to a different respective modulation regime. An emission detector detects emissions of fluorescent radiation from the samples. The emission detector is adapted to detect each of said different respective modulation regimes in emitted radiation such that responses of the samples within the multiple vessels to said at least two sources can be separately detected.