MONOCLONAL ANTIBODY DIRECTED AGAINST THE HUMAN LDL RECEPTOR

摘要

Monoclonal antibody directed against human low-density lipoprotein receptor comprises light chain variable regions encoded by a defined murine nucleic acid sequence, heavy chain variable regions encoded by a defined murine nucleic acid sequence, and light and heavy chain constant regions derived from a nonmurine species. Monoclonal antibody directed against human low-density lipoprotein receptor comprises light chain variable regions encoded by murine nucleic acid sequence SEQ ID NO:5 (defined sequence of 336 nucleotides given in the specification), heavy chain variable regions encoded by murine nucleic acid sequence SEQ ID NO:7 (defined sequence of 345 nucleotides given in the specification), and light and heavy chain constant regions derived from a nonmurine species. Independent claims are also included for: (1) vector for expressing the light chain of an antibody as above, with SEQ ID NO:12 (defined sequence of 6288 nucleotides given in the specification); (2) vector for expressing the heavy chain of an antibody as above, with SEQ ID NO:18 (defined sequence of 6717 nucleotides given in the specification); (3) stable cell line producing the antiibody; (4) DNA fragment with SEQ ID NO:19 (defined sequence of 1341 nucleotides given in the specification) coding for the heavy chain of the antibody; (5) DNA fragment with SEQ ID NO:13 (defined sequence of 663 nucleotides given in the specification) coding for the light chain of the antibody; (6) use of a monoclonal antibody directed against human low-density lipoprotein receptor for preparing a medicament for treating melanoma. ACTIVITY : Cytostatic. MECHANISM OF ACTION : Immunotherapy.