| 摘要 |
A disposable chromatography column is provided, which includes: a chromatography column body having an external wall and a hollow chamber through the body from an inlet end to an outlet end of the body, a shoulder adjacent the outlet end of the chromatography column body with a first fritted disk received on the shoulder, a flange extending radially outward from the inlet end of the chromatography column body, chromatographic media filling at least a portion of the chamber of the chromatography column body with a second fritted disk sitting on top of the chromatographic media furthest from the outlet end of the chromatography column body, a piston having an externally threaded upper portion, a lower portion smaller in diameter than the upper portion inserted into the inlet end of the chromatography column body, an annular recess on the lower portion of the piston with a sealing element located in the recess, a through-bore or conduit extending through the upper and lower portions of the piston for receiving and providing liquid to the second fritted disk and the chromatographic media in the chamber of the chromatography column body, and a retainer having first and second ends with an opening extending from the first end to the second end of the retainer, the opening at the first end sized to snugly slide over and engage the external wall of the chromatography column body, an annular shoulder between the first and second ends of the retainer and abutting the opening at the first end of the retainer, the annular shoulder receiving and supporting the annular flange extending from the chromatography column body, the opening having internal threads adjacent the opening at the second end of the retainer, the internal threads engaging the external threads of the piston whereby screwing the external threads of the piston into the internal threads of the retainer forces the lower portion of the piston and the annular sealing element against the second fritted disk in the chamber of the chromatography column body.
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