The invention relates to the use of PCR to detect a blown pack spoilage-causing strain of Clostridia in a meat product. The strain of Clostridia is characterised by having a target sequence in the 16S rRNA gene (SEQUENCE ID NO: 1) which is shown to be unique and capable of distinguishing the strain from other sources of blown pack spoilage and other sources of meat spoilage. The invention also provides a primers and probes for detection of the target sequence, and a PCR kit for specific detection of the strain.
申请公布号
WO2010032233(A2)
申请公布日期
2010.03.25
申请号
WO2009IE00066
申请日期
2009.09.17
申请人
AGRICULTURE AND FOOD DEVELOPMENT AUTHORITY (TEAGASC);BOLTON, DECLAN;BURGRESS, KATHERINE;SHERIDAN, JAMES
发明人
BOLTON, DECLAN;BURGRESS, KATHERINE;SHERIDAN, JAMES