| 摘要 |
<P>PROBLEM TO BE SOLVED: To provide a vector used for both a cell-free protein synthesis system and a baculovirus expression system. <P>SOLUTION: The baculovirus transfer vector comprises a baculovirus late gene promoter sequence (a), a baculovirus late gene 5' untranslated region sequence (b), and a sequence (c) for inserting a desired structural gene from the upstream side to the downstream side, and further comprises a phage-derived RNA polymerase recognition sequence (d) at the upstream side of the sequence (a) or the downstream side of the sequence (a) and at the upstream side of the sequence (b). The method for preparing a protein with a baculovirus expression system and/or a cell-free protein synthesis system from a recombinant baculovirus transfer vector obtained by inserting a desired structural gene to the baculovirus transfer vector is provided. <P>COPYRIGHT: (C)2010,JPO&INPIT |
