METHOD FOR IDENTIFYING IRRITATING AND ALLERGENIC SUBSTANCES

摘要

The present invention relates to a method for identifying irritating or allergenic potential of a product comprising the steps of: a) contacting the product with immature dendritic cells, b) determining the amount of 65 gene products, expressed in said cells upon contact with the product, c) subtracting the amount of gene products of these genes expressed in said cells without contact with the product from the respective amounts determined under b) to obtain a set of product-caused amounts of gene products for each of said genes, d) comparing the product-caused amounts of gene products for each of said genes obtained under c) with a test set of product-caused amounts of gene products for each of said genes, wherein said test set of product-caused amounts of gene products for each of said genes was provided from at least two allergenic products and at least two irritating products and, optionally, from at least two products which are neither allergenic nor irritating; and wherein the comparing is performed by a pattern recognition method which recognises an allergenic pattern and an irritating pattern and, optionally, a non-allergenic and non-irritating pattern; and e) identifying an allergenic potential of a product when the comparison under d) results in the recognition of an allergenic pattern and identifying an irritating potential of a product when the comparison under d) results in the recognition of an irritating pattern and, optionally, identifying a non-allergenic and non-irritating product when the comparison under d) results in the recognition of a non-allergenic and non-irritating pattern.