发明名称 |
Direct ATP release sequencing |
摘要 |
A method for sequencing a nucleic acid is provided. In certain embodiments, the method includes contacting a nucleic acid duplex comprising a nucleic acid template and a primer annealed to the template with a reagent mix under primer extension conditions to produce an extended primer and ATP. The reagent mix may contain an adenosine-2′-deoxynucleoside tetraphosphate moiety and a polymerase. The method further includes detecting the produced ATP. Also provided are adenosine-2′-deoxynucleoside tetraphosphate moieties that find use in the subject methods. Also provided are kits containing the adenosine-2′-deoxynucleoside tetraphosphate moieties for use in the subject methods.
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申请公布号 |
US7682809(B2) |
申请公布日期 |
2010.03.23 |
申请号 |
US20080013377 |
申请日期 |
2008.01.11 |
申请人 |
AGILENT TECHNOLOGIES, INC. |
发明人 |
SAMPSON JEFFREY R. |
分类号 |
C12P19/34;C07H21/04;C12Q1/68;G01N15/06 |
主分类号 |
C12P19/34 |
代理机构 |
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主权项 |
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地址 |
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