METHODS FOR DETECTING AND QUANTIFYING OVERSULFATED GLYCOSAMINOGLYCANS
摘要
<p>The invention relates to novel methods for detecting and/or quantifying oversulfated or persulfated glycosaminoglycans based on inhibition of nucleic acid polymerases. The methods can be utilized to detect and quantify oversulfated or persulfated glycosaminoglycans in pharmaceutical preparations, such as heparin preparations or therapeutic medical devices. When used to detect or quantify oversulfated glycosaminoglycans in heparin containing solutions, the samples are prepared by treatment with heparinases to degrade the heparin. Titration of the inhibition of the activity of the polymerases allows quantitation of the oversulfated glycosaminoglycans in the sample.</p>
申请公布号
WO2010030608(A1)
申请公布日期
2010.03.18
申请号
WO2009US56263
申请日期
2009.09.08
申请人
THE GOVERNMENT OF THE UNITED STATES OF AMERICA ASREPRESENTED BY THE SECRETARY OF THE DEPARTMENT OFHEALTH AND HUMAN SERVICES;VERTHELYI, DANIELA;TAMI, MARIA, CECILIA;PUIG, MONTSERRAT