Method to eliminate polymerase chain reaction inhibitors from feacal samples

摘要

The present invention relates to the field of sample preparation methods and kits for PCR diagnosis of gastroenteritis. Disclosed is a method for removing polymerase chain reaction (PCR) inhibitory substances from faecal samples and disrupting bacterial cells to be assayed prior to PCR by using a two-phase aqueous polymer system and by subjecting the cells to ultrasonic energy.