发明名称 |
Method for amplifying genomic DNA |
摘要 |
A method for amplifying genomic DNA is provided. The method comprises the steps of: (1) incubating a cell-containing agarose solution at a pH of 9 to 12 and a temperature of 45 to 80° C. to produce a genomic DNA-dispersed agarose solution wherein 0.002 to 1 copies/5 microliter of single-stranded genomic DNA is dispersed; (2) solidifying the genomic DNA-dispersed agarose solution to produce a genomic DNA-dispersed agarose gel and neutralizing a pH of the gel; and (3) adding a DNA polymerase with strand displacement activity, primer and dNTP to the genomic DNA-dispersed agarose gel and incubating the gel at a temperature of 0 to 60° C. to amplify the genomic DNA.
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申请公布号 |
US7678543(B2) |
申请公布日期 |
2010.03.16 |
申请号 |
US20080214044 |
申请日期 |
2008.06.16 |
申请人 |
NATIONAL INSTITUTE OF RADIOLOGICAL SCIENCES |
发明人 |
IMAI TAKASHI;IWAKAWA MAYUMI;MICHIKAWA YUICHI |
分类号 |
C12Q1/68;C12P19/34 |
主分类号 |
C12Q1/68 |
代理机构 |
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地址 |
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