发明名称 |
Methods and compositions for the detection of a nucleic acid using a non-invasive cleavage reaction |
摘要 |
The invention provides methods, compositions and kits for generating a signal indicative of the presence of a target nucleic acid sequence in a sample comprising forming a cleavage structure by incubating a sample comprising a target nucleic acid sequence with upstream and downstream oligonucleotides, and cleaving the cleavage structure with a nuclease to generate a signal. The presence of a detectable signal is indicative of the presence of a target nucleic acid sequence and a non-invasive cleavage structure.
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申请公布号 |
US7678541(B2) |
申请公布日期 |
2010.03.16 |
申请号 |
US20060639781 |
申请日期 |
2006.12.15 |
申请人 |
HOLOGIC, INC. |
发明人 |
SORGE JOSEPH A.;HAPPE SCOTT;FIRMIN ANDREW |
分类号 |
C12Q1/68;C07H21/04;C12P19/34 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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