| 摘要 |
A method for differentiating a human mesenchymal stem cell which is positive to CD73, CD90, CD105, and CD166 to osteoblast is provided to shorten differentiation time and have stability in use. An osteroblast is differentiated by culturing a human mesenchymal stem cell which is positive to CD73, CD90, CD105, and CD166 in medium with a fucoidan. The concentration of fucoidan is 0.1-5 mg/ml. The medium obtained by adding the fucoidan comprises the fucoidan, fetal bovine serum(FBS), dexamethasone, beta-glycerophospyhate, and low-glucose DMEM (Dulbecco's Modified Eagle Medium ) having ascorbic acid. The mesenchymal stem cell is bone marrow, umbilical cord blood, or adipose-derived mesenchymal stem cell. |
