NOVEL LIPID-GOLD NANOPARTICLE HYBRID, AND GENE DELIVERY METHOD USING THE SAME

摘要

PURPOSE: A method for delivery of a physiological active substance using a novel lipid-gold nanoparticle complex is provided to improve delivery efficiency and to minimize cytotoxicity. CONSTITUTION: A gold nanoparticle complex contains gold nanoparticle physiological active substance conjugated to the surface of the gold nanoparticle. The physiological active substance is DNA, RNA, plasmid DNA, peptide, protein, polynucleotide or oligonucleotide. The complex further contains a polyethylene glycol(PEG) conjugated at the surface of the gold nanoparticle. A method for preparing the complex comprises: a step of mixing the gold nanoparticle and physiological active substance; a step of binding the physiological active substance on the surface of the gold nanoparticle by electrostatic interaction; and a step of encapsulating the gold nanoparticle and physiological substance with lipid.