HIGH-PURITY PURIFICATION METHOD FOR OMEGA-3 HIGHLY UNSATURATED FATTY ACIDS

摘要

The present invention relates to a high-purity purification method for omega-3 highly unsaturated fatty acids. More specifically, it relates to a high-purity purification method for omega-3 highly unsaturated fatty acids which is both environmentally friendly and easy to implement and which comprises the steps of: a) preparing a fatty acid ethyl ester (FAEE) by ethanolysis of a natural oil or fat, using ethanol, in the presence of an enzyme catalyst extracted from at least one microorganism selected from the group consisting of Candida genus, Rhizopus genus, Mucor genus, Aspergillus genus and Pseudomonas genus; b) subjecting the said prepared fatty acid ethyl ester to preliminary distillation using short-path distillation (SPD) at between 100 and 200°C and between 0.001 and 10mmHg; c) forming a concentrated fatty acid by subjecting the ethyl ester, which has been subjected to the preliminary distillation, to reduced-pressure fractional distillation at between 100 and 200°C and between 0.001 and 10mmHg; and d) purifying the concentrated fatty acid by means of simulated moving bed (SMB) column chromatography.