| 摘要 |
The present invention provides an antibody-based multiplex detection method for identifying one or more epitopes in one or more samples, the method comprising the steps of: (a) contacting each of the samples comprising the epitopes with one or more antibodies specific to the epitopes to be detected; (b) washing unbound antibodies away and eluting bound antibodies from the epitope carriers to provide separate antibody eluates; and (c) detecting and quantifying antibodies in the antibody eluates to generate separate profiles of antibodies for each of the samples. The epitopes to be detected can be located intracellularly, or present on the surface of cells or an artificial surface. In general, the epitopes are derived from protein, polypeptides with or without post-translational modification, carbohydrate, nucleic acid, or lipid.
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