| 摘要 |
The present invention aims to provide a three-dimensional cell culture method for increasing cell proliferation efficiency by suitably regulating the proliferation-inducing and proliferation-inhibitory signals between cells, which are the greatest variables in proliferation efficiency. For this purpose, the present invention provides a method for culturing cells using micro-scaffolds by the regulation of cell-to-cell signals, the method comprising repeatedly performing any one or both of the following processes a) and b) so as to regulate proliferation-inducing and proliferation- inhibitory signals between the cells: a) a process of gradually adding the micro-scaffolds, in which a small amount of the micro-scaffolds are used in an initial stage in order to maintain a suitable distance between the cells (cell concentration relative to the micro-scaffolds), and the amount of the micro-scaffolds is then increased slowly according to cell proliferation rate! and b) a periodic shaking process, in which shaking is performed in order to separate connected cells from each other by a physical force, after the cells are incubated for more than a given period of time.
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