METHOD OF PRODUCING CHONDROITIN-6-SULPHATE FROM UMBILICAL CORDS OF NEWLY BORN BABIES

摘要

FIELD: chemistry. ^ SUBSTANCE: mixture of chondroitin-6-sulphate and dermatan sulphate extracted from umbilical cords is oxidised with 2,2,6,6-tetramethylpiperidine-1-oxyl-NaClO-NaBr at pH 10.2 and temperature 0-5C for 1 hour with subsequent precipitation of oxidation products with three ethanol objects and their separation on a chromatographic column with DEAE-cellulose using eluent in form of aqueous solutions of NaCl with increasing concentration 0.2åÆ0.6 M in increment of 0.05. ^ EFFECT: obtaining pure chondroitin-6-sulphate; the rest of the products are hybrid polysaccharides consisting of chondroitin-6-sulphate and dermatan sulphate oxidised on the primary groups. ^ 1 ex