METHOD OF EVALUATING IRRITATING ACTION AND ACTIVITY OF NATURAL, SYNTHETIC SUBSTANCES AND READY PREPARATIONS ON CHICK EMBRYOS BY METHOD OF ULTRASONIC DOPPLEROGRAPHY

摘要

FIELD: medicine. ^ SUBSTANCE: method relates to experimental and clinical pharmacology. Analysed substance is applied in layers on choriallantoic membrane of 10-day chick embryo and injury of membrane vessels is evaluated. Evaluation of change of blood flow rate and heart rate is carried out in dynamics by means of ultrasonic Dopplerography. Ultrasound sensor is first placed on the surface of choriallantoic membrane at angle to examined venous vessel, and after that is submerged into albumen to located on yolk sac arteries, at angle to them, and venous and arterial Dopplerograms are obtained respectively. Control measurement is performed before applying layer of analysed substance. By obtained Dopplerograms linear rate of blood flow Vs and heart rate are determined and hypo- or hypertensive impact of substance is evaluated by their change. ^ EFFECT: invention allows to increase accuracy and objectivity of investigation results interpretation for wide range of medications, increase economy and quickness of obtaining results. ^ 18 tbl, 8 ex