| 摘要 |
<p>The invention discloses a target specific double-mutant fusion protein, which is constructed from a fusion protein of human gonadotropin-releasing hormone mutant (mGnRH) and recombinant Pseudomonas aeruginosa exotoxin A mutant (PE38m4a). The mGnRH-PE38m4a protein of the present invention has significant specific binding activity and cytotoxicity to tumor cell lines including colon cancer HT-29 cells, ovarian cancer OVCAR3 cells, cervical adenocarcinoma HeLa cells and liver cancer HepG-2 cells, and the like; and has essentially no lethal effect to normal cells, and has a 9-10 fold higher activity than natural PEA, and is low-cost, easy to produce and suitable for industrial produce.</p> |
