HIGH-THROUGHPUT AND NON-INVASIVE METHOD TO VITRIFY PORCINE EMBRYOS
摘要
<p>The present invention provides a practical, non-invasive, and efficient method for cryopreservation of an in-vitro-produced porcine embryo. The inventive method treats the IVP (such as IVF- or NT-derived) embryo at the one-cell or cleavage stage prior to compaction with high osmolality followed by high speed centrifugation. The high osmolality treatment enlarges the periviteline space, and with centrifugation, enables the separation of the lipids from the cytoplasm. The lipid-separated embryos after high osmolality treatment have been successfully cryopreserved and later recovered and transferred to produce live offspring.</p>
申请公布号
WO2010025404(A1)
申请公布日期
2010.03.04
申请号
WO2009US55424
申请日期
2009.08.28
申请人
THE CURATORS OF THE UNIVERSITY OF MISSOURI;PRATHER, RANDALL, S.;LI, RONGFENG;MURPHY, CLIFTON, N.;SPATE, LEE
发明人
PRATHER, RANDALL, S.;LI, RONGFENG;MURPHY, CLIFTON, N.;SPATE, LEE