发明名称 |
High-throughput and non-invasive method to vitrify porcine embryos |
摘要 |
The present invention provides a practical, non-invasive, and efficient method for cryopreservation of an in-vitro-produced porcine embryo. The inventive method treats the NP (such as IVF- or NT-derived) embryo at the one-cell or cleavage stage prior to compaction with high osmolality followed by high speed centrifugation. The high osmolality treatment enlarges the periviteline space, and with centrifugation, enables the separation of the lipids from the cytoplasm. The lipid-separated embryos after high osmolality treatment have been successfully cryopreserved and later recovered and transferred to produce live offspring. |
申请公布号 |
AU2009285530(A1) |
申请公布日期 |
2010.03.04 |
申请号 |
AU20090285530 |
申请日期 |
2009.08.28 |
申请人 |
THE CURATORS OF THE UNIVERSITY OF MISSOURI |
发明人 |
RANDALL S. PRATHER;RONGFENG LI;CLIFTON N. MURPHY;LEE SPATE |
分类号 |
C12N5/02;A01K67/00;A61D19/04 |
主分类号 |
C12N5/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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