MICROPATTERNING PROCESS FOR NEURAL NETWORKS USING THREE-DIMENSIONAL STRUCTURES

摘要

PURPOSE: A neuron chip capable of patterning neural cells using three dimensional physical structure and a method for patterning are provided to easily form neural cell network and produce microelectrode array(MEA) chip. CONSTITUTION: A method for patterning neural cells in a neuron chip using three dimensional PDMS structure comprises: a first process of manufacturing a silicon master substrate having plural three dimensional recesses; a second process of pouring PDMS mixture solution on a silicon master substrate to form PDMS substrate having lane and plural three dimensional structure; a third process of separating PDMS substrate from the silicon master substrate; and a fourth process of culturing neural cells on the PDMS substrate to obtain neuron chip in which at least one neural cell is patterned.