发明名称 |
A DNA REPLICON SYSTEM FOR HIGH-LEVEL RAPID PRODUCTION OF VACCINES AND MONOCLONAL ANTIBODY THERAPEUTICS IN PLANTS |
摘要 |
Plant viral vectors have great potential in rapid production of proteins, but no simple Here a geminivirus-based system for high-yield and rapid production of oligomeric protein complexes, including virus-like particle (VLP) vaccines and monoclonal antibodies (mAbs) is described. In particular, a single vector that contains two non-competing replicons for transient expression in Nicotiana benthamiana leaves is described. The correct assembly of these subunit proteins into functional oligomeric structures (VLPs or full-size mAb) is also described. This system advances plant transient expression technology by eliminating the need for non-competing viruses, and thus, enhances the realistic commercial application of this technology for producing multiple-subunit protein complexes. |
申请公布号 |
CA2736796(A1) |
申请公布日期 |
2010.03.04 |
申请号 |
CA20092736796 |
申请日期 |
2009.08.27 |
申请人 |
ARIZONA BOARD OF REGENTS FOR AND ON BEHALF OF ARIZONA STATE UNIVERSITY |
发明人 |
MASON, HUGH S.;HUANG, ZHONG;CHEN, QIANG;ARNTZEN, CHARLES J.;YUAN, SHUO;HJELM, BROOKE |
分类号 |
C12N15/82;A01H5/00;C12N15/00;C12N15/54 |
主分类号 |
C12N15/82 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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