发明名称 |
A NOVEL VECTOR AND EXPRESSION CELL LINE FOR MASS PRODUCTION OF RECOMBINANT PROTEIN AND A PROCESS OF PRODUCING RECOMBINANT PROTEIN USING SAME |
摘要 |
<p>Disclosed herein is an inducible high-expression cassette comprising a dihydrofolate reductase (DHFR) promoter from which GC-rich repeat sequences are partially or entirely removed, the cassette capable of more effectively improving a gene amplification system. Also disclosed are an expression vector comprising the inducible expression cassette and optionally a gene encoding a recombinant protein of interest, an animal cell line transformed with the expression vector, and a method of mass producing and purifying a recombinant protein by culturing the transformant. The present invention enables the shortening of the time required to establish a cell line producing a recombinant protein of interest at high levels using a low concentration of a DHFR inhibitor, thereby allowing more effective production of the recombinant protein.</p> |
申请公布号 |
EP2087118(A4) |
申请公布日期 |
2010.03.03 |
申请号 |
EP20070833373 |
申请日期 |
2007.10.16 |
申请人 |
HANMI PHARMACEUTICAL. CO., LTD. |
发明人 |
CHOI, IN YOUNG;KIM, CHANG HWAN;LEE, HYUN JI;PARK, SEONG HEE;KWON, SE CHANG;LEE, GWAN SUN |
分类号 |
C12N15/63;C12N15/09;C12N15/79 |
主分类号 |
C12N15/63 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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