发明名称 |
OXIDATIVELY CROSS-LINKED PROTEIN-BASED ENCAPSULATES |
摘要 |
The present invention provides a method of producing a protein-based encapsulate, said method comprising: providing an aqueous solution of a protein that is capable of forming disulfide cross-links; submitting said aqueous solution to a protein activation treatment to produce an aqueous suspension of activated protein aggregates, said suspension having a reactivity of at least 5.0 mumol thiol groups per gram of activated protein aggregates as determined in the Ellman's assay; dispensing said aqueous suspension in a gas or a water-immiscible liquid to produce suspension droplets having a diameter of 0.1-500 mum; and forming disulfide cross-links between the activated protein aggregates by contacting the activated protein aggregates with an oxidizing agent, optionally after said activated protein aggregates have been partially cross-linked by forming disulfide cross-links by means of heat treatment or by pressurization to a pressure in excess of 50 MPa. The aforementioned method offers the advantage that the characteristics of the protein-based encapsulation matrix can be controlled effectively. Furthermore, said method enables the preparation of protein-based encapsulates that very effectively protect the encapsulated components, e.g. against oxidation or moisture. |
申请公布号 |
EP2158032(A2) |
申请公布日期 |
2010.03.03 |
申请号 |
EP20080753781 |
申请日期 |
2008.05.21 |
申请人 |
NIZO FOOD RESEARCH B.V. |
发明人 |
ALTING, AART CORNELIS;FLORIS, THEODORUS ARNOLDUS GERARDUS;WEINBRECK, FANNY CHANTAL JACQUELINE;GRANDIA, JEROEN;VAN DE VELDE, FREDDIE;BODNAR, IGOR |
分类号 |
B01J13/04;A23L1/22;A23P1/04 |
主分类号 |
B01J13/04 |
代理机构 |
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