发明名称 |
Process for producing modified reconstituted sendai viral envelope specific for drug and/or gene delivery to liver cells |
摘要 |
A process for producing a targeted gene and/or drug delivery system for liver cells comprising the steps of: i. chemical reduction of Sendai virus for reduction of HN protein; ii. subjecting the reduced virus to the step of dialysis for removal of the reducing agent; iii. solubilizing the reduced virus with a detergent to obtain a solution; iv. centrifuging the solution to separate the insolubles consisting of reduced HN protein and core of said virus; v. adding histidylated lipid to the supernatant; vi. adding the drug or gene after addition of the lipid; vii. removing the detergent from the envelope and then subjecting it to the step of centrifugation to obtain His lipid F-virosomes with entrapped drug or DNA.
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申请公布号 |
US2010047897(A1) |
申请公布日期 |
2010.02.25 |
申请号 |
US20060918841 |
申请日期 |
2006.02.24 |
申请人 |
VERMA SANTOSH K;SHARMA NISHI RAJ;KUMAR VALLURIPALLI VINOD;CHAUDHURI ARABINDA;SARKAR DEBI P;MANI PRASHANT;KRISHNAN ANUJA;REDDY BATHULA SURENDAR;ROY RAJANDRA P |
发明人 |
VERMA SANTOSH K;SHARMA NISHI RAJ;KUMAR VALLURIPALLI VINOD;CHAUDHURI ARABINDA;SARKAR DEBI P.;MANI PRASHANT;KRISHNAN ANUJA;REDDY BATHULA SURENDAR;ROY RAJANDRA P. |
分类号 |
C12N7/06 |
主分类号 |
C12N7/06 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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