| 摘要 |
The present invention relates to a novel nucleotide probe for detecting nucleic acids and constituted of a labelled nucleotide strand comprising three fragments: a first fragment having a first closing sequence, a second fragment of which all or part has a recognition sequence for molecular recognition of the target nucleic acid, a third fragment having a second closing sequence, and at least two labels, one of the ends of the strand of said detection probe being free of any label, in which, when the two closing sequences are hybridized together, the detection probe has a completely circular shape, said closing sequences thus maintaining said probe in an undetectable conformation in the absence of said target nucleic acid. The invention also relates to the detection method and kit using this probe. The invention is for preferential use in the diagnostics field. |
